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gsk 3484862  (MedChemExpress)


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    Structured Review

    MedChemExpress gsk 3484862
    Gsk 3484862, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gsk+3484862/GSK-3484862/pm42231006-256-8-9
    Average 95 stars, based on 43 article reviews
    gsk 3484862 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Methylation:

    Article Title: A maternal exon H splice-site variant leading to pseudohypoparathyroidism type 1B with broad methylation defects in GNAS-differentially methylated regions.
    Article Snippet: Images were obtained using an LSM510 laser scanning confocal microscope (Zeiss). .. DNA demethylation using DNMT1 inhibitor To demethylate methylated CpGs, GSK-3484862 (MedChemExpress), a selective DNMT1 inhibitor, was dissolved in dimethyl sulfoxide and applied to the culture medium at a final concentration of 10 μM, following previously reported methods [25, 40]. .. After seven days incubation, we extracted gDNA and total RNA from iPSCs using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen).

    Concentration Assay:

    Article Title: A maternal exon H splice-site variant leading to pseudohypoparathyroidism type 1B with broad methylation defects in GNAS-differentially methylated regions.
    Article Snippet: Images were obtained using an LSM510 laser scanning confocal microscope (Zeiss). .. DNA demethylation using DNMT1 inhibitor To demethylate methylated CpGs, GSK-3484862 (MedChemExpress), a selective DNMT1 inhibitor, was dissolved in dimethyl sulfoxide and applied to the culture medium at a final concentration of 10 μM, following previously reported methods [25, 40]. .. After seven days incubation, we extracted gDNA and total RNA from iPSCs using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen).

    Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome.
    Article Snippet: Cells were irradiated in a UV irradiation chamber (Opsytec Dr. Gröbel, BS-02) with an intensity of 8 mW cm−2 for a final dose of 10 J cm−2 (around 18 min) controlled by a dosimeter. .. For DNMT1 inhibition experiment with HAP1 and U2OS cells, 10 ml of of cell culture medium was supplemented with 4 μl of GSK-3484862 (MedChemExpress, HY-135146; 10 mM, dissolved in DMSO; final concentration: 4 μM). ..

    Inhibition:

    Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome.
    Article Snippet: Cells were irradiated in a UV irradiation chamber (Opsytec Dr. Gröbel, BS-02) with an intensity of 8 mW cm−2 for a final dose of 10 J cm−2 (around 18 min) controlled by a dosimeter. .. For DNMT1 inhibition experiment with HAP1 and U2OS cells, 10 ml of of cell culture medium was supplemented with 4 μl of GSK-3484862 (MedChemExpress, HY-135146; 10 mM, dissolved in DMSO; final concentration: 4 μM). ..

    Article Title: DAXX promotes SUMOylation of chromatin-trapped DNMT1
    Article Snippet: .. For DNMT1 inhibition, egg extracts were incubated with 50 μM 5-aza-dCTP (Jena Bioscience) or with 100 μM GSK-3484862 (MedChemExpress). .. For the inhibition of the SUMOylation pathway, egg extracts were incubated with UBC9-dominant negative, which were kindly gifted from Dr Azuma (the University of Kansas) or with 50 μM ML-792 (MedChemExpress).

    Article Title: Mechanisms of gene regulation by SRCAP and H2A.Z
    Article Snippet: .. For DNMT1 inhibition, SYAT cells were treated with 2 μM GSK-3484862 (MedChemExpress #HY-135146) for 5 days before performing ATAC-seq. .. All used cell lines were tested every 6 months for the absence of Mycoplasma by Eurofins Genomics.

    Article Title: DAXX promotes SUMOylation of chromatin-trapped DNMT1.
    Article Snippet: .. 298 299 For DNMT1 inhibition, egg extracts were incubated with 50 μM 5-aza-dCTP (Jena Bioscience) 300 or with 100 μM GSK-3484862 (MedChemExpress). .. For the inhibition of the SUMOylation 301 pathway, egg extracts were incubated with UBC9-dominant negative, which were kindly 302 gifted from Dr Azuma (the University of Kansas) or with 50 μM ML-792 (MedChemExpress).

    Cell Culture:

    Article Title: Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome.
    Article Snippet: Cells were irradiated in a UV irradiation chamber (Opsytec Dr. Gröbel, BS-02) with an intensity of 8 mW cm−2 for a final dose of 10 J cm−2 (around 18 min) controlled by a dosimeter. .. For DNMT1 inhibition experiment with HAP1 and U2OS cells, 10 ml of of cell culture medium was supplemented with 4 μl of GSK-3484862 (MedChemExpress, HY-135146; 10 mM, dissolved in DMSO; final concentration: 4 μM). ..

    Incubation:

    Article Title: DAXX promotes SUMOylation of chromatin-trapped DNMT1
    Article Snippet: .. For DNMT1 inhibition, egg extracts were incubated with 50 μM 5-aza-dCTP (Jena Bioscience) or with 100 μM GSK-3484862 (MedChemExpress). .. For the inhibition of the SUMOylation pathway, egg extracts were incubated with UBC9-dominant negative, which were kindly gifted from Dr Azuma (the University of Kansas) or with 50 μM ML-792 (MedChemExpress).

    Article Title: DAXX promotes SUMOylation of chromatin-trapped DNMT1.
    Article Snippet: .. 298 299 For DNMT1 inhibition, egg extracts were incubated with 50 μM 5-aza-dCTP (Jena Bioscience) 300 or with 100 μM GSK-3484862 (MedChemExpress). .. For the inhibition of the SUMOylation 301 pathway, egg extracts were incubated with UBC9-dominant negative, which were kindly 302 gifted from Dr Azuma (the University of Kansas) or with 50 μM ML-792 (MedChemExpress).



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